Rapid and economical protocols for genomic and metagenomic DNA extraction from oak (Quercus brantii Lindl.)
نویسندگان
چکیده
منابع مشابه
A Rapid and Economical Method for Efficient DNA Extraction from Diverse Soils Suitable for Metagenomic Applications
A rapid, cost effective method of metagenomic DNA extraction from soil is a useful tool for environmental microbiology. The present work describes an improved method of DNA extraction namely "powdered glass method" from diverse soils. The method involves the use of sterile glass powder for cell lysis followed by addition of 1% powdered activated charcoal (PAC) as purifying agent to remove humic...
متن کاملA Simple and Rapid Leaf Genomic DNA Extraction Method for Polymerase Chain Reaction Analysis
In plants, secondary metabolites and polysaccharides interfere with genomic isolation procedures and downstream reactions such as restriction enzyme analysis and gene amplification. The removal of such contaminants needs complicated and time-consuming protocols. In this study, a simple, rapid and efficient method for leaf DNA extraction was optimized. This method use small amount of plant mater...
متن کاملEconomical and Rapid Method for Extracting Cotton Genomic DNA
A fast, simple, and reliable mini-prep method for the extraction of DNA from Gossypium species and cultivars has been developed. This small-scale method is cetyltrimethylammonium bromide (cTAB)-based, and it extracts DNA from one to three folded or nearly unfolded young leaves processed in a 1.5 mL tube with 0.5 mL of extraction buffer and homogenized by an electric drill. Compared with the mac...
متن کاملEffect of Simulated Dust Storm on some Bio-chemical features of Persian Oak (Quercus brantii Lindl.)
Extended abstract 1- Introduction Dust storm is a hazardous natural event affecting all creatures. (1). Due to the global warming drought, reduction in precipitation, and mismanagement in the water by humankind, the negative effects of this calamity were more observed (2). With increasing the wind speed, and based on the size of dust particles, land topography, soil humidity, vegetation cover...
متن کاملEffect of Hydro-alcoholic Extract of Persian Oak (Quercus brantii) in Experimentally Gastric Ulcer
Persian oak (Quercus brantii Lindl.) belongs the family Fagaceae, is a medicinal plant which seed flour is used to treat inflammatory and gastric ulcers by the tribes in south western Iran. The current study was done to evaluate the effect of hydro-alcoholic extract of Q. brantii seed flour for treatment of gastric ulcers induced by ethanol in Wistar rats. The hydro-alcoholic extract of Q. bran...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Annals of Forest Science
سال: 2018
ISSN: 1286-4560,1297-966X
DOI: 10.1007/s13595-018-0705-y